Effects of Vitamin C, E and K Supplementation on Hematological Indices and Serum Iron in Female Wistar Rats

Abstract

Background: Vitamins are indispensable micronutrients that support the regulation and optimization of blood cell production, function and hematological health. This experimental study investigates the effect of vitamins C, E and K on hematological indices and serum iron levels in female Wistar rats.

Methods: Forty rats were randomized into 8 groups of five rats each. Group 1 as control was given only food and water. Groups 2-4 (100mg/kg, 500mg/kg, and 10mg/kg body weight of vitamin C, E and K). Group 5 (100mg/kg and 500mg/kg body weight of vitamin C and E), group 6 (100mg/kg and 10mg/kg body weight of vitamin C and K), group 7 (500mg/kg and 10mg/kg of body weight of vitamin E and K), group 8 (100mg/kg, 500mg/kg and 10mg/kg body weight of vitamin C, E and K). These rats were orally administered these vitamins and had food and water also for one month. Blood was collected by cardiac puncture for analysis.

Results: there was significant (P<0.05) decrease in total WBC in vitamin C and C+E groups,  significant (P<0.05) increase in neutrophil count in vitamin E, C+E and  C+E+K groups with a significant (P<0.05) increase in neutrophil/lymphocyte ratio in vitamin C, C+E and C+E+K groups. A significant (P<0.05) decreased in platelet-lymphocyte ratio in control group. Vitamin C+E group had a significantly (P<0.05) increased PLR. Vitamin E+K and C+E+K had a significant (P<0.05) increased serum iron level.

Conclusion: the overall result shows the regulatory effect of these vitamins on optimal blood health.